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. 2020 Mar 3;11:237. doi: 10.3389/fpls.2020.00237

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Copyright © 2020 Betterle, Hidalgo Martinez and Melis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) SDS-PAGE and Coomassie-staining analysis of Synechocystis wild type, CpcB^∗IFN, and CpcB^∗His^∗IFN total cell extract, and of proteins eluted from the resin column upon application of 250 mM imidazole. (B) Western blot analysis with specific IFN polyclonal antibodies of the proteins resolved in (A). Note the heterologous ∼36 kD CpcB^∗His^∗IFN and the ∼108 kD putative CpcB^∗His^∗IFN trimer (marked by arrowheads).