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. 2020 Mar 3;11:237. doi: 10.3389/fpls.2020.00237

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Copyright © 2020 Betterle, Hidalgo Martinez and Melis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Map of the nptI^∗IFN fusion construct in the cpc operon locus. Note the presence of the His-tag and the Xa protease cleavage site in-between the two genes in the fusion. (B) SDS-PAGE and Coomassie staining of the protein extracts from wild type (WT), the cpcB^∗His^∗IFN, and two independent lines of the nptI^∗His^∗IFN transformants. (C) Western blot analysis of a duplicate gel as the one shown in (B). Specific anti-IFN polyclonal antibodies were used in this analysis. Note the specific antibody cross reactions with protein bands migrating to ∼36 kD (CpcB^∗His^∗IFN) and ∼46 kD (NptI^∗His^∗IFN). Also note the antibody cross reactions with protein bands of higher molecular mass.