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. 2020 Mar 3;11:237. doi: 10.3389/fpls.2020.00237

FIGURE 4.

FIGURE 4

Protein expression analysis of Synechocystis wild type and transformants. (A) Total cellular protein extracts were resolved by SDS-PAGE and visualized by Coomassie-stain. Two independent replicates of total protein extracts from wild type (WT), and IFN, CpcB-IFN, and CpcB∗IFN transformant cells were loaded onto the SDS-PAGE. Individual native and heterologous proteins of interest are indicated to the right of the gel. Sample loading corresponds to 0.25 μg of chlorophyll. Note the clear presence of a heterologous protein migrating to ∼36 kD in the CpcB∗IFN fusion extracts. (B) Total protein extracts of (A) were subjected to Western-blot analysis with loading of the lanes as per Figure (A). Specific polyclonal antibodies against the human IFN protein were used to probe target proteins. Sample loading corresponds to 0.25 μg of chlorophyll. Note the specific antibody cross-reaction with proteins migrating to ∼36 and ∼108 kD in the cpcB∗IFN fusion and the absence of a cross reaction with any protein from the IFN and cpcB-IFN transformant cells. The latter do not seem to make/accumulate IFN.