FIGURE 5.

Protein expression analysis of Synechocystis wild type (WT) and transformants harboring the cpcB^∗IFN fusion construct. Total cellular protein extracts were resolved by SDS-PAGE and visualized by Coomassie-stain. Two different versions of the IFN gene were used: the human native IFN’ and the Synechocystis codon-optimized IFN gene. Note the presence of heterologous proteins migrating to ∼36 kD (CpcB^∗IFN) and ∼23 kD (CmR) in the transformants but not in the wild type. Also note the presence of the ∼19 kD CpcB β-subunit and the ∼17 kD CpcA α-subunit of phycocyanin in the wild type but not in the transformants. Sample loading corresponds to 0.5 μg of chlorophyll. Quantification of the CpcB^∗IFN protein accumulation relative to that of the Rubisco large subunit (RbcL) is given in the results of Table 1.