FIGURE 6.

Protein expression analysis of Synechocystis wild type (WT) and transformants harboring the cpcB^∗His^∗IFN fusion construct. Total cellular protein extracts were resolved by SDS-PAGE and visualized by Coomassie-stain. Two different versions of the fusion construct were used comprising the CpcB^∗IFN fusion and the more extensive cpcB^∗His^∗IFN fusion configuration, followed by the cmR resistance cassette. Equivalent amount of the CpcB^∗IFN and the CpcB^∗His^∗IFN fusion proteins were expressed in Synechocystis. Individual native and heterologous proteins of interest are indicated to the right of the gel. Sample loading corresponds to 0.25 μg of chlorophyll.