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Effect of miRNA-125a-5p on the Expression of EGFR in A549 Cells. The EGFR expression determined by RT-qPCR at 24, 48 and 72 h after transfection of the cells with miRNA-125a-5p and negative control (NC) miRNA. Relative EGFR mRNA expression was measured using the 2- (∆∆Ct) method. The EGFR mRNA decreased clearly at the three time points compared with corresponding blank control and NC miRNA groups (*p<0.05). The results presented are mean±SD of three independent experiments. #p <0.05
