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. 2020 Mar 9;10:4363. doi: 10.1038/s41598-020-61381-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Doxycycline decreases oxidative phosphorylation. (a) Brown histochemical staining for cytochrome-c oxidase activity in fibroblasts, A549 and A549 ρ° cells treated with vehicle or doxycycline for 5 days. Cell nuclei were counterstained purple with haematoxylin. Scale bar represents 10 μm (b,c) Mean cytochrome-c oxidase and citrate synthase activity in lysates of cells treated with vehicle (Veh) or doxycycline (DC) for 5 days (n ≥ 4). (d) Mean basal respiration, maximal respiration, spare respiratory capacity and respiration coupled to ATP production in cells treated with vehicle or doxycycline for 5 days (n ≥ 5). (e) Mean basal glycolysis, glycolytic capacity and glycolytic reserve in cells treated with vehicle or doxycycline for 5 days (n ≥ 5). Error bars indicate standard deviations. Asterisks denote statistically significant differences (*p < 0.05; **p < 0.01).