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. 2020 Mar 9;11(3):177. doi: 10.1038/s41419-020-2379-2

Fig. 3. S63845 induces apoptosis by targeting MCL1 to disrupt the MCL1:BAK complex.

Fig. 3

a Immunoblotting analysis of lysates from DMS114 and KTOR201 following siRNA-mediated MCL1 knockdown, which affected MCL1, BCL-XL, and BCL-2 expression. b Cell growth assays following siRNA-mediated MCL1 knockdown using two different MCL1-directed RNAi sequences (A and B) in DMS114 and KTOR201. Relative cell viability was measured 72 h after transfection with siRNA using CellTiter-Glo. Results are expressed as mean ± SEM (N = 16). One-way ANOVA with Tukey’s multiple comparison test was used. *p < 0.05. c Immunoblotting analysis of lysates from DMS114 and KTOR201 following siRNA-mediated BAK1, BAX, or double knockdown affecting BAK and BAX expression. d Cell growth assays after 72 h of exposure to 100 nM S63845 following siRNA-mediated BAK1, BAX, or double knockdown. Results are expressed as mean ± SEM (N = 5). One-way ANOVA with Tukey’s multiple comparison test was used. *p < 0.05 versus siNC group. e DMS114 and KTOR201, treated or not-treated with S63845, were subjected to anti-MCL1 immunoprecipitation. The input (100%) and immunoprecipitants were assessed by immunoblot analysis. *p < 0.05.