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. 2020 Mar 9;11(3):177. doi: 10.1038/s41419-020-2379-2

Fig. 4. Knockdown of BCL-XL and BCL-2 improves the cytotoxic activity of S63845 in S63845-resistant cell lines.

Fig. 4

a Cell growth assays following siRNA-mediated knockdown of MCL1, BCL-XL, or both in SW1271, and that of MCL1, BCL-XL, BCL-2, or all in DMS53 using two different RNAi sequences (A and B). Relative cell viability measured 72 h after transfection with siRNA using CellTiter-Glo. Results are expressed as mean ± SEM (N = 16, SW1271; N = 36, DMS53). One-way ANOVA with Tukey’s multiple comparison test was used. *p < 0.05 versus siNC group. b Immunoblotting analysis of lysates from SW1271 and DMS53 following siRNA-mediated knockdown of MCL1, BCL-XL, BCL-2, or all affecting MCL1, BCL-XL, and BCL-2 expression. c Cell viability assay of SW1271 transfected with BCL-XL siRNA or negative control siRNA treated with S63845 72 h after transfection. Cell viability assay of DMS53 transfected with BCL-XL, BCL-2, or both siRNA, or negative control siRNA treated with S63845 72 h after transfection. Results are expressed as mean ± SEM (N = 5). Two-way ANOVA with Tukey’s multiple comparison test demonstrated a significant difference between siNC and RNAi sequence A (*) and between siNC and RNAi sequence B (†) for each S63845 concentration. *, †p < 0.05.