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. 2020 Mar 3;11:180. doi: 10.3389/fphar.2020.00180

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Copyright © 2020 Mahdi, Georges, Ali, Bedeer, Eltahry, Gabr and Berger

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Messenger RNA (mRNA) expression of endothelin system members endothelin-1 (EDN1), endothelin-2 (EDN2), endothelin-3 (EDN3), endothelin-converting enzyme 1 (ECE1), endothelin-converting enzyme 2 (ECE2), endothelin receptor A (EDNRA), and endothelin receptor (EDNRB) in two human (HT29 and SW620) and in rat CC531 colorectal cancer cells after exposure to decitabine (DAC, 5 µM) either as a single administration (second row) or as a daily administration over 3 days (third row) in comparison to untreated control cells (first row) as detected by real-time (RT)-PCR. The housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control.