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. 2020 Mar 3;11:180. doi: 10.3389/fphar.2020.00180

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Copyright © 2020 Mahdi, Georges, Ali, Bedeer, Eltahry, Gabr and Berger

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA expression of endothelin system members (EDN1, EDN2, EDN3, ECE1, ECE2, EDNRA, and EDNRB) as detected by real-time (RT)-PCR in human (SW480) and rat (CC531) colorectal cancer cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene. (A) Decitabine (DAC) at a constant concentration (4 µM), but varied exposure time [two (second row), or three (third row), or four (fourth row) daily administrations]. (B) Decitabine (DAC) with three increasing concentrations but constant exposure period (0.5, 1.0, 2.0 µM in SW480 and 0.25, 0.5, 1.0 µM in CC531 cells; administered once daily for 3 days).