Table 1.

Expression of EDN1 mRNA in SW480 cells after daily exposure to decitabine as shown by real-time (RT)-PCR.

DAC concentration	ΔCT a)	ΔΔCT b)	Fold change c)
2 × daily (4 µM)	13.59	−2.93	7.64
3 × daily (4 µM)	11.32	−6.54	93.27
4 × daily (4 µM)	10.85	−5.68	51.15
3 × daily (0.25 µM)	16.02	−1.84	3.57
3 × daily (0.5 µM)	14.75	−3.11	8.61
3 × daily (1 µM)	12.85	−5.01	32.30
3 × daily (2 µM)	11.6	−6.26	76.64

^a)ΔC_T= C_T (EDN1)-C_T (GAPDH) (C_T; threshold cycle: the PCR cycle at which the fluorescent signal of the reporter dye crosses an arbitrarily placed threshold).

^b)ΔΔC_T= ΔC_T (treated sample)-ΔC_T (untreated sample).

^c)Fold change= 2^{−(∆∆Ct)}.