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. 2020 Jan 25;57(1):88–96. doi: 10.2141/jpsa.0190049

Fig. 3.

Fig. 3.

Expression of albumin (ALB) in the utero-vaginal junction (UVJ). (a) Gene expression of ALB in the UVJ. The mRNA extracted from the UVJ was reverse transcribed, and an aliquot was subjected to PCR using an ALB-specific primer set (lane +). As a non-reverse transcribed control, mRNA from the UVJ was treated the same way, except that reverse transcriptase (lane -) was omitted. A representative gel of three independent experiments is shown. (b) The UVJ extracts (0.1 µg protein/lane) were separated by SDS–PAGE under reducing conditions, transblotted onto PVDFmembranes, and detected with anti-ALB antiserum (lane -, 1:10,000) or anti-ALB antiserum preabsorbed with antigen protein (lane +, 1: 10,000). Immunohistochemical observations using anti-ALB antiserum (c) or anti-ALB antiserum preabsorbed with antigen protein (d). Nuclei were counterstained with hematoxylin. Representative results of three experiments are shown. Bar=50 µm. (e) An oviductal flush was prepared and 0.1 µg protein/lane was detected with anti-ALB antiserum (lane -, 1:10,000) or anti-ALB antiserum preabsorbed with antigen protein (lane +, 1:10,000).