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Effects of transferrin (TF) and albumin (ALB) on the longevity of ejaculated sperm. Ejaculated sperm were suspended in HBSS supplemented with various concentrations of (a) purified TF(0, 25, 100, and 175 µg/mL) or (b) purified ALB (0, 50, 225, or 450 µg/mL) and incubated for 24 h at 4°C. After 24 h of incubation, a sperm viability assay was performed. Data shown are for three independent experiments. The number of sperm counted per plot ranged from 758 to 1,432.
