Figure 13.

The role of mitochondrial Ca²⁺ uptake on T lymphocyte activation. Agonist was applied from 200 s to 1200 s. [Ca²⁺]_o was held at 2,0 mM throughout the simulation. (A) Block of the channel conductance of the mitochondrial Ca²⁺ uniporter (red) slightly increases the [Ca²⁺]_cyt transient compared to control (black). Blocking the Ca²⁺-dependent inhibition of ORAI1 (blue) or blocking the Ca²⁺-dependent rate of inhibition (green) result in a large [Ca²⁺]_cyt transient. (B) Block of the channel conductance of the mitochondrial Ca²⁺ uniporter (red) slightly decreases NFAT translocation to the nucleus compared to control (black). Blocking the Ca²⁺-dependent inhibition of ORAI1 (blue) or blocking the Ca²⁺-dependent rate of inhibition (green) increases NFAT translocation to the nucleus. (C) Block of the channel conductance of the mitochondrial Ca²⁺ uniporter (red) slightly decreases CRAC current compared to control (black). Blocking the Ca²⁺-dependent inhibition of ORAI1 (blue) or blocking the Ca²⁺-dependent rate of inhibition (green) result in increased CRAC current.