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. 2020 Feb 25;2020:9349132. doi: 10.1155/2020/9349132

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Copyright © 2020 Takehiro Matsubara et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Correlation between RNA quality indexes and NGS library yields. (a, b) Correlation between RINe and DV200 of all samples in terms of amount of the 1^st NGS library product per input (◇: fresh, ●: frozen, and ×: FFPE). NGS libraries were prepared using TruSeq RNA Access or TruSight RNA Pan-Cancer. The amount of the 1^st NGS library product was measured using Qubit. Threshold lines are drawn for the amount of the 1^st NGS library product (10 ng/ng) and the cutoff value (RINe: 2.3 and DV200: 66.1) on the basis of receiver operating characteristic (ROC) curve analysis. (c)–(f) are segregated from all samples depending on sample type: fresh sample (◇: c, d) or FFPE sample (×: e, f).