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CIRBP inhibited Aβ25-35-induced neurotoxicity via the antioxidative pathway. (a) Intracellular ROS level was measured using a ROS-detecting fluorescence dye DCF-DA kit. (b) The activities of oxidative stress biomarkers, such as 4-HNE and MDA, measured by ELISA assay. (c) The activities of key enzymes in an antioxidant system, such as SOD, CAT, and GPx, were measured by ELISA. ∗∗p < 0.01vs. Ad-Con, ##p < 0.01vs. Ad-Con+Aβ25-35.
