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. 2020 Feb 27;2020:9342732. doi: 10.1155/2020/9342732

Figure 5.

Figure 5

GLPG1790 induces senescence in HCT116 cells via the activation of ERK signaling. (a) Cell lysates from HCT116 and HCT15 cells treated with 2 μM and 7 μM GLPG1790, respectively, for 24 or 48 h were analyzed using immunoblotting with specific antibodies for indicated proteins. α-Tubulin expression functions as a loading control. (b) β-Galactosidase activity was assessed by ELISA using HCT116 and HCT15 cells that had been cotreated with U0126 (10 μM) and GLPG1790, 2 μM or 7 μM, respectively, for 36 h (∗∗∗P < 0.001 vs. untreated, §§§P < 0.001 vs. GLPG1790). (c) Cell lysates from HCT116 and HCT15 cells cotreated with U0126 (10 μM) and GLPG1790, 2 μM or 7 μM, respectively, for 36 h were analyzed by immunoblotting with specific antibodies for indicated proteins. α-Tubulin expression functions as a loading control.