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. 2020 Feb 25;2020:9349762. doi: 10.1155/2020/9349762

Figure 1.

Figure 1

Cell viability, apoptosis, and ROS production of NPMSCs with H2O2 treatment. (a) Cell viability of NPMSCs treated with different concentrations (0, 40, 80, 120, and 160 μM) of H2O2 for 24 hours. (b) Cell viability of NPMSCs treated with 120 μM of H2O2 for different times (0, 1, 12, and 24 hours). (c) TUNEL staining of NPMSCs (bar = 50 μm). (d) Representative images of cell apoptosis detected by flow cytometry after Annexin V/PI dual staining. (e) Summary data showing the apoptosis rate in different groups. The apoptotic cells were stained with Annexin V+/PI- and Annexin V+/PI+. (f) ROS production evaluated by fluorescence staining and flow cytometry (bar = 100 μm). (g) Summary data showing the level of ROS in different groups. NS means no significant difference. The data are expressed as the mean ± SD from three independent experiments (∗∗P < 0.01, ∗∗∗P < 0.001 vs. 0 μM of H2O2).