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. 2020 Feb 18;8(3):1636–1648. doi: 10.1002/fsn3.1450

Figure 6.

Figure 6

Effect of pretreatment with finger citron crude flavonoids (FCCF) on reactive oxygen species (ROS) and malondialdehyde (MDA) accumulation, enzyme activity, and resistance to stress in Caenorhabditis elegans. After 96 hr of treatment with FCCF or control, 200 worms were collected for sample preparation. ROS (a) and MDA (b) accumulation was measured according to the Materials and Methods 2.4.6 and 2.4.7. SOD (c) and CAT (d) activity was expressed as units/mg of protein. Values with different letters are significantly different (p < .05 for “a” comparisons “b” or “A” comparisons “B”). ##The C. elegans were exposed to 25 mM Paraquat. Worms were pretreated with FCCF and survived significantly longer after 37°C heat shock (e; N ≥ 30 animals per group) and exposure to 25 mg/ml paraquat (f; N ≥ 30 animals per group). Three independent trials were performed. Data are expressed as the mean ± SD of three independent experiments (N = 3)