Figure 5.

The disruption of MMP‐9/Hsp90 interaction by honokiol through the inhibition of HDAC6 activity in H1299 cells. (a) H1299 cells were treated with honokiol (0, 2.5, 5, 7.5, 10 μM) for 24 hr. After treatment, Western blot analyses were used to identify the expression of HDAC6, acetyl‐α‐tubulin, Hsp90, and β‐actin. (b) H1299 cells were treated with 10 μM of honokiol for 24 hr, and then, immunoprecipitation analysis was performed. The immunoprecipitates of anti‐acetyllysine and MMP‐9 were then revealed by Western blotting. (c) H1299 cells were incubated with 2 μM of tubacin or 10 μM of honokiol for 24 hr. Afterward, cells were collected and the expression of HDAC6, acetyl‐α‐tubulin, and MMP‐9 were determined by Western blot analyses. Data were represented by the mean ± SD of triplicate samples. Significant difference was compared with the control group (*, p < .05)