Table 3.
Concentration (µg TOS/plate) | Revertant colonies per plate (mean ± SD) | |||||||
---|---|---|---|---|---|---|---|---|
TA100 | TA1535 | TA98 | TA1537 | |||||
−S9 | +S9 | −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | |
Negative control (distilled water)a | 136 ± 9 | 150 ± 16 | 13 ± 3 | 22 ± 2 | 40 ± 3 | 57 ± 4 | 5 ± 1 | 17 ± 3 |
803 | 140 ± 6 | 153 ± 9 | 11 ± 0 | 13 ± 3 | 40 ± 3 | 46 ± 4 | 6 ± 2 | 21 ± 3 |
1,610 | 145 ± 7 | 152 ± 4 | 8 ± 3 | 13 ± 3 | 37 ± 0 | 51 ± 2 | 6 ± 2 | 14 ± 2 |
3,210 | 131 ± 8 | 131 ± 7 | 11 ± 2 | 17 ± 2 | 34 ± 2 | 43 ± 2 | 4 ± 1 | 13 ± 2 |
6,430 | 124 ± 5 | 145 ± 8 | 13 ± 1 | 13 ± 1 | 43 ± 5 | 45 ± 3 | 7 ± 2 | 15 ± 2 |
12,850 | 118 ± 9 | 135 ± 6 | 14 ± 4 | 17 ± 3 | 48 ± 5 | 42 ± 4 | 7 ± 1 | 13 ± 2 |
Positive controlb, c | 700 ± 59 | 412 ± 30 | 62 ± 4 | 132 ± 11 | 302 ± 11 | 239 ± 27 | 554 ± 19 | 91 ± 4 |
Abbreviations: −S9, in the absence of S9; +S9, in the presence of S9; 2‐AA, 2‐aminoanthracene; 4‐NQO, 4‐nitroquinoline 1‐oxide; 9‐AA, 9‐aminoacridine hydrochloride; AF‐2, 2‐(2‐furyl)‐3‐(5‐nitro‐2‐furyl) acrylamide; GT, glutaminase from nongenetically modified A. niger strain GT147; SD, standard deviation; TOS, total organic solids.
100 µl/plate.
Positive control −S9: TA100 = 0.02 µg/plate AF‐2; TA1535 = 0.5 µg/plate 4‐NQO; TA98 = 0.05 µg/plate AF‐2; TA1537 = 5.0 µg/plate 9‐AA.
Positive control + S9: TA100 = 1.0 µg/plate 2‐AA; TA1535 and TA1537 = 2.0 µg/plate 2‐AA; TA98 = 0.5 µg/plate 2‐AA.