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. 2020 Feb 18;8(3):1433–1450. doi: 10.1002/fsn3.1426

Table 3.

Results of main bacterial reverse mutation test of GT using the treat‐and‐wash method

Concentration (µg TOS/plate) Revertant colonies per plate (mean ± SD)
TA100 TA1535 TA98 TA1537
−S9 +S9 −S9 +S9 −S9 +S9 −S9 +S9
Negative control (distilled water)a 136 ± 9 150 ± 16 13 ± 3 22 ± 2 40 ± 3 57 ± 4 5 ± 1 17 ± 3
803 140 ± 6 153 ± 9 11 ± 0 13 ± 3 40 ± 3 46 ± 4 6 ± 2 21 ± 3
1,610 145 ± 7 152 ± 4 8 ± 3 13 ± 3 37 ± 0 51 ± 2 6 ± 2 14 ± 2
3,210 131 ± 8 131 ± 7 11 ± 2 17 ± 2 34 ± 2 43 ± 2 4 ± 1 13 ± 2
6,430 124 ± 5 145 ± 8 13 ± 1 13 ± 1 43 ± 5 45 ± 3 7 ± 2 15 ± 2
12,850 118 ± 9 135 ± 6 14 ± 4 17 ± 3 48 ± 5 42 ± 4 7 ± 1 13 ± 2
Positive controlb, c 700 ± 59 412 ± 30 62 ± 4 132 ± 11 302 ± 11 239 ± 27 554 ± 19 91 ± 4

Abbreviations: −S9, in the absence of S9; +S9, in the presence of S9; 2‐AA, 2‐aminoanthracene; 4‐NQO, 4‐nitroquinoline 1‐oxide; 9‐AA, 9‐aminoacridine hydrochloride; AF‐2, 2‐(2‐furyl)‐3‐(5‐nitro‐2‐furyl) acrylamide; GT, glutaminase from nongenetically modified A. niger strain GT147; SD, standard deviation; TOS, total organic solids.

a

100 µl/plate.

b

Positive control −S9: TA100 = 0.02 µg/plate AF‐2; TA1535 = 0.5 µg/plate 4‐NQO; TA98 = 0.05 µg/plate AF‐2; TA1537 = 5.0 µg/plate 9‐AA.

c

Positive control + S9: TA100 = 1.0 µg/plate 2‐AA; TA1535 and TA1537 = 2.0 µg/plate 2‐AA; TA98 = 0.5 µg/plate 2‐AA.