Table 6.
Results of the confirmative in vivo mammalian alkaline comet assay conducted in male rats orally administered GT once daily for 2 days
| Substance Dose (mg TOS/kg) | Number of animals | Duodenum | |
|---|---|---|---|
| % tail DNAa (mean ± SD) | Frequency of hedgehogs (%) (mean ± SD) | ||
| Negative control (distilled water; 20 ml/kg) | 5 | 1.70 ± 0.24 | 0.5 ± 1.2 |
| 1,260 | 5 | 1.96 ± 0.40 | 0.4 ± 0.4 |
| 1,800 | 5 | 1.94 ± 0.55 | 0.8 ± 1.1 |
| 2,570 | 5 | 1.76 ± 0.21 | 0.4 ± 0.6 |
| 2,570b | 1.73 ± 0.16 | 0.7 ± 0.7 | |
| Positive control (EMS; 200 mg/kg) | 5 | 26.34 ± 1.56* | 2.1 ± 1.7 |
Abbreviations: EMS, ethyl methanesulfonate; GT, glutaminase from nongenetically modified A. niger strain GT147; SD, standard deviation; TOS, total organic solids.
a
The mean % tail DNA in each group was calculated from that in each animal based on the median % tail DNA in each slide.
b
Inactivated enzyme.
*
Significantly different from negative control (Aspin–Welch's t test, p ≤ .025).