Table 7.
Hematology values for male and female rats administered GT by gavage for 13 weeks
| Parameter measured (mean ± SD) | Dose group (mg TOS/kg body weight) | |||||||
|---|---|---|---|---|---|---|---|---|
| Males (n = 10) | Females (n = 10) | |||||||
| 0 (control)a | 161 | 643 | 2,570 | 0 (control)a | 161 | 643 | 2,570 | |
| Hematocrit (%) | 46.1 ± 2.0 | 46.2 ± 1.3 | 45.3 ± 1.4 | 46.4 ± 1.5 | 44.6 ± 1.9 | 43.8 ± 1.3 | 44.2 ± 1.7 | 43.2 ± 1.4 |
| Hemoglobin (g/dl) | 15.5 ± 0.7 | 15.6 ± 0.5 | 15.5 ± 0.5 | 15.6 ± 0.4 | 15.5 ± 0.5 | 15.2 ± 0.6 | 15.2 ± 0.7 | 15.0 ± 0.5 |
| RBC (×106/mm3) | 8.64 ± 0.42 | 8.68 ± 0.18 | 8.60 ± 0.34 | 8.61 ± 0.27 | 8.21 ± 0.35 | 7.98 ± 0.28 | 8.14 ± 0.38 | 7.93 ± 0.26 |
| MCV (µM3) | 53.3 ± 1.2 | 53.2 ± 1.2 | 52.7 ± 1.1 | 53.9 ± 1.3 | 54.3 ± 0.9 | 54.9 ± 1.0 | 54.3 ± 0.9 | 54.6 ± 1.0 |
| MCH (pg) | 18.0 ± 0.4 | 18.0 ± 0.5 | 18.0 ± 0.4 | 18.1 ± 0.5 | 18.8 ± 0.3 | 19.1 ± 0.4 | 18.7 ± 0.3 | 19.0 ± 0.4 |
| MCHC (%) | 33.8 ± 0.3N | 33.8 ± 0.3 | 34.1 ± 0.2 | 33.7 ± 0.7 | 34.7 ± 0.5 | 34.7 ± 0.5 | 34.4 ± 0.3 | 34.8 ± 0.3 |
| Reticulocyte ratio (%) | 2.3 ± 0.6N | 2.1 ± 0.2 | 2.3 ± 0.5 | 2.1 ± 0.3 | 2.0 ± 0.2 | 2.2 ± 0.4 | 1.6 ± 0.3* | 2.1 ± 0.4 |
| Reticulocyte count (×109/L) | 195.5 ± 41.7N | 186.3 ± 15.0 | 199.6 ± 38.7 | 178.0 ± 21.7 | 165.6 ± 18.6 | 171.6 ± 26.6 | 131.8 ± 22.0* | 165.3 ± 32.3 |
| WBC (×103/mm3) | 8.27 ± 1.45 | 8.51 ± 2.29 | 7.35 ± 1.92 | 8.91 ± 2.22 | 4.58 ± 1.10 | 4.64 ± 1.32 | 5.18 ± 1.78 | 4.55 ± 1.59 |
| Differential leukocyte ratios (%) | ||||||||
| Neutrophil (%) | 17.4 ± 5.4 | 19.6 ± 8.0 | 18.4 ± 4.8 | 19.3 ± 10.3 | 15.5 ± 5.1 | 16.3 ± 5.1 | 13.0 ± 3.6 | 13.1 ± 5.9 |
| Lymphocyte (%) | 76.7 ± 6.3 | 74.6 ± 8.2 | 76.3 ± 4.7 | 75.8 ± 10.4 | 78.4 ± 6.0 | 77.6 ± 5.6 | 80.6 ± 4.0 | 81.3 ± 6.4 |
| Monocyte (%) | 3.5 ± 1.1 | 3.3 ± 0.7 | 2.9 ± 1.0 | 2.9 ± 0.6 | 2.6 ± 1.0 | 3.2 ± 0.9 | 2.9 ± 0.8 | 2.6 ± 0.7 |
| Eosinophil (%) | 1.5 ± 0.4 | 1.8 ± 0.6 | 1.6 ± 0.6 | 1.3 ± 0.3 | 2.5 ± 0.8N | 2.0 ± 0.4 | 2.5 ± 0.7 | 2.1 ± 1.1 |
| Basophil (%) | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.0 | 0.1 ± 0.1 | 0.1 ± 0.0 |
| LUC (%) | 0.8 ± 0.3 | 0.6 ± 0.2 | 0.7 ± 0.3 | 0.7 ± 0.3 | 1.0 ± 0.3 | 0.8 ± 0.2 | 0.9 ± 0.5 | 0.8 ± 0.3 |
| Differential leukocyte counts (×108/mm3) | ||||||||
| Neutrophil (×103/mm3) | 1.41 ± 0.42N | 1.69 ± 0.83 | 1.30 ± 0.28 | 1.82 ± 1.50 | 0.69 ± 0.22 | 0.76 ± 0.34 | 0.64 ± 0.19 | 0.60 ± 0.38 |
| Lymphocyte (×103/mm3) | 6.38 ± 1.42 | 6.33 ± 1.81 | 5.66 ± 1.65 | 6.64 ± 1.51 | 3.62 ± 1.04 | 3.60 ± 1.04 | 4.20 ± 1.56 | 3.69 ± 1.32 |
| Monocyte (×103/mm3) | 0.28 ± 0.07 | 0.28 ± 0.09 | 0.22 ± 0.11 | 0.26 ± 0.11 | 0.12 ± 0.05 | 0.15 ± 0.05 | 0.15 ± 0.06 | 0.12 ± 0.06 |
| Eosinophil (×103/mm3) | 0.12 ± 0.04 | 0.15 ± 0.07 | 0.11 ± 0.04 | 0.12 ± 0.04 | 0.12 ± 0.04 | 0.09 ± 0.03 | 0.12 ± 0.02 | 0.10 ± 0.05 |
| Basophil (×103/mm3) | 0.01 ± 0.01 | 0.01 ± 0.01 | 0.01 ± 0.00 | 0.01 ± 0.01 | 0.00 ± 0.01 | 0.00 ± 0.00 | 0.01 ± 0.01 | 0.01 ± 0.01 |
| LUC (×103/mm3) | 0.07 ± 0.03 | 0.05 ± 0.02 | 0.06 ± 0.03 | 0.07 ± 0.04 | 0.05 ± 0.02 | 0.04 ± 0.02 | 0.05 ± 0.03 | 0.04 ± 0.02 |
| Blood coagulation analysis | ||||||||
| Platelet (×103/mm3) | 1,002 ± 187N | 1,008 ± 94 | 1,053 ± 82 | 1,044 ± 70 | 1,097 ± 110 | 1,107 ± 134 | 1,110 ± 178 | 1,092 ± 114 |
| PT (seconds) | 12.2 ± 1.1 | 12.2 ± 1.2 | 12.9 ± 1.6 | 10.9 ± 1.0 | 8.9 ± 0.3 | 8.9 ± 0.2 | 8.9 ± 0.1 | 8.8 ± 0.2 |
| APTT (seconds) | 23.5 ± 2.0 | 22.5 ± 2.9 | 24.7 ± 1.8 | 21.7 ± 2.4 | 15.6 ± 2.3 | 16.4 ± 1.6 | 16.6 ± 2.1 | 16.2 ± 1.4 |
Abbreviations: APTT, activated partial thromboplastin time; GT, glutaminase from nongenetically modified A. niger strain GT147; LUC, large unstained cells; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; MCV, mean corpuscular volume; N, nonparametric analysis; PT, prothrombin time; RBC, red blood cell; SD, standard deviation; TOS, total organic solids; WBC, white blood cell.
a
Control animals were administered water.
*
Significantly different from control (Dunnett's multiple test, p ≤ .05).