Isik 2000.
Methods | Single centre, randomised trial | |
Participants | 46 women from Turkey with > 5 day 3 cleavage stage embryos (FSH at day 3: control 6.1 (3.0); AH 5.5 (1.4) IU/L)undergoing ICSI Mean duration of infertility: 6.7 years Mean age: control group 29.1 (3.6); AH group 30.5 (5.2) years | |
Interventions | AH enzymatic (chemical; complete and total zona breach; 120 to 144 hours egg retrieval to AH; 0.5 to 1 hours AH to transfer) versus no AH AH: 24 women randomised, 71 embryos transferred Control: 22 women randomised, 63 embryos transferred | |
Outcomes | Implantation | |
Notes | Author response No. of embryos transferred, blastocyst transfer: AH 2.95 (0.9); Control 2.86 (0.8) Unclear if power calculation performed ITT analysis unclear Published as full paper |
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Risk of bias | ||
Bias | Authors' judgement | Support for judgement |
Random sequence generation (selection bias) | Low risk | Using a random number table on day three |
Allocation concealment (selection bias) | High risk | Allocation not concealed |
Blinding (performance bias and detection bias) All outcomes | Unclear risk | Participants not blinded or unclear Assessor not blinded or unclear |
Incomplete outcome data (attrition bias) All outcomes | Unclear risk | Not stated |
Selective reporting (reporting bias) | Unclear risk | Protocol not viewed, however live birth was not reported |