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. 2019 Nov 2;68(2):425–429. doi: 10.1136/jim-2019-001124

Figure 4.

Figure 4

MiR-3150b-3p directly targeted Golgi phosphoprotein 3 (GOLPH3) in colorectal cancer (CRC) cells. (A) Alignment of miR-3150b with GOLPH3 3′-UTR sequences. HEK293T cells were co-transfected with luciferase reporter with wild-type (WT) GOLPH3 3′-UTR or with mutant GOLPH3 3′-UTR, and miR-3150b-3p mimic or mimic NC for 48 hours. The relative luciferase activity was analyzed by luciferase assay. The mRNA (B) and protein (C) levels of GOLPH3 in HCT116 cells transfected with miR-3150b-3p mimic or mimic NC, and SW480 cells transfected with miR-3150b-3p inhibitor or inhibitor NC, using qRT-PCR and western blotting. *p<0.05; **p<0.01 versus mimic NC or inhibitor NC group.