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. 2020 Jan 16;118(5):1067–1075. doi: 10.1016/j.bpj.2019.12.037

Figure 2.

Figure 2

Thermal unfolding of mAb in 1.0 M guanidineHCl at pH 6.2, measured with differential scanning calorimetry (DSC). Shown is molar heat capacity Cp(T) as a function of temperature. Black line: experimental result. Smooth lines: simulations with the multistate Zimm-Bragg theory (green: pretransition; blue: main transition; red: sum of pre- and main transition). Pretransition parameters: Tm = 54°C, ΔHcal = 322 kcal/mol (ν = ΔHcal/h = 293); ΔCp =5.02 kcal/mol⋅K, σ = 1.5 × 10−4. Main transition parameters: Tm = 75.4°C, ΔHcal = 976 kcal/mol (ν = 887); ΔCp =12.42 kcal/mol⋅K, σ = 7 × 10−5.