Figure 5.

Unfolding enthalpy ΔH_cal as a function of midpoint temperature T_m. Red squares, blue squares, and green squares represent main transitions at pH 4.0, 6.2, and 8.0, respectively. Slope ${\text{C}}_{{\text{pT}}_{\text{0}}}$ = 16.8 ± 1.6 kcal/mol∙K. Red circles, blue circles, and green circles represent pretransitions at pH 4.0, 6.2, and 8.0, respectively. Slope ${\text{C}}_{{\text{pT}}_{\text{0}}}$ = 8.0 ± 0.5 kcal/mol∙K.