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. 2020 Mar 3;12:36. doi: 10.3389/fnagi.2020.00036

Figure 2.

Figure 2

(A) Gating scheme for identification of pDC (Lin-HLA DR+CD123+) in the peripheral blood. (B) Characterization of SQM plasmacytoid dendritic cells (pDCs) and analysis of CpG ODN effect on Lin-HLA DR+ and Lin-CD123+ expressing cells. Aliquots of the whole blood were stained with the Lin1-FITC antibody cocktail consisting of FITC-labeled antibodies to CD3, CD14, CD16, CD20, PE-labeled antibodies to CD123, and PerCP-labeled antibodies to HLA DR to identify pDCs. Values on the Y-axis represent % expression of Lin1-HLA DR+ and Lin-CD123+ cells evaluated in the presence (5 μg/ml and 50 μg/ml) or absence (unstimulated) of CpG ODN. P-values were considered statistically significant at p < 0.05. (C) Gating scheme for identification of CD123 expression on major lymphocyte and monocyte subsets in the peripheral blood. (D) Analysis of CD123 expression on major lymphocyte and monocyte subsets in the peripheral blood from a CpG ODN injected SQM. Aliquots of the whole blood were stained with antibodies to B cells (CD20+; a), T cells (CD3+; b), NK cells (CD3-CD16+; c), and NKT cells (CD3+CD16+; d) to identify lymphocyte subpopulations and stained with antibodies to identify monocytes (CD14+; e) at day 0, 1, 3, and 7. Values on the Y-axis are % expressions of CD123 marker on lymphocyte and monocyte cell populations. Symbols representing significance are: ***p < 0.001; **p < 0.01; *p < 0.05; ns, p > 0.05.