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Viability assay. For viability of PBMCs we used a standard MTT proliferation dye reduction assay. PBMCs were cultured in triplicate wells at 1 × 105/well in 96-well round-bottomed plates with 5 μg/ml and 50 μg/ml Class C CpG ODN or 5 μg/ml concanavalin A (Con A) for 96 h at 37°C. Proliferation responses were measured as optical density (OD) and data are presented as % viability over cells without stimulation. Symbols representing significance are: ****p < 0.0001.
