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. 2020 Mar 3;11:164. doi: 10.3389/fphar.2020.00164

Figure 4.

Figure 4

Knockdown of QKI abates the effects of miR-362-5p inhibition on the proliferation of bladder cancer cells. (A, B) The SW780 cells were co-transfected with miR-362-5p inhibitor/NC inhibitor (50 pmol) and QKI siRNA/NC siRNA (50 pmol) for 48 h. Then the cell proliferation and cell viability were determined by staining BrdU (bar=50 μm) and MTT assay. The cell viability was displayed as fold of NC inhibitor. (C) The cell cycle distribution of the transfected cells was measured using flow cytometer after 48 h transfection. The percentage of cells was displayed as fold of NC inhibitor in G1 phase. (D) The protein levels of QKI, Cyclin D, MacroH2A1.1, MacroH2A1.2, p27, c-Fos, PARP-1, and E2F1 in the transfected cells were measured by western blot analysis after 48 h transfection. GAPDH was used as an internal control in western blot. **p < 0.01 and *p < 0.05 vs. corresponding controls.