Figure 2.

Sequence Modification Prevents Formation of Homodimer-like Structures

(A) Propensity to form homodimer-like structures predicted with the OligoAnalyzer tool from IDT. The | symbol is used to depict Watson-Crick base pairing and the : symbol for wobble base pairing. For H51(+67)W, the most stable structure predicted by the OligoAnalyzer tool is shown in (Aa) and the original structure now containing wobble in (Ab). (B) Electrophoresis of tcDNA-ASOs in nondenaturing acrylamide gel to evaluate their ability to form homodimer-like structures. (C) Representation of the hybridization of tcDNA-ASO on the pre-mRNA target. H51(+67) tcDNA fully hybridizes with targeted pre-mRNA through Watson-Crick pairing (left); H51(+67)W tcDNA hybridizes with targeted pre-mRNA through one wobble pairing underlined (right).