Skip to main content
. 2019 Nov 16;19:905–921. doi: 10.1016/j.omtn.2019.10.043

Figure 7.

Figure 7

PKP2 Was Regulated by LINC00680, TTN-AS1, miR-320b, and EGR3

(A) Western blot analysis of PKP2 in U87 and U251 cell knockdown of LINC00680 or TTN-AS1. Data are presented as the mean ± SD (n = 3 in each group). *p < 0.05 versus sh-NC group. (B) Western blot analysis of PKP2 in U87 and U251 cells regulated by LINC00680/TTN-AS1 and miR-320b. Data are presented as the mean ± SD (n = 3 in each group). **p < 0.01 versus sh-NC+pre-NC group. (C) Western blot analysis of PKP2 in U87 and U251 cells regulated by miR-320b. Data are presented as the mean ± SD (n = 3 in each group). **p < 0.01 versus pre-NC group; ##p < 0.01 versus anti-NC group. (D) Western blot analysis of PKP2 in U87 and U251 cells regulated by miR-320b and EGR3. Data are presented as the mean ± SD (n = 3 in each group). **p < 0.01 versus anti-NC group; ##p < 0.05 versus anti-miR-320b group. (E) Western blot analysis of PKP2 in U87 and U251 cells regulated by EGR3. Data are presented as the mean ± SD (n = 3 in each group). **p < 0.01 versus sh-NC group. (F) Putative EGR3 binding sites are indicated. Immunoprecipitated DNA was amplified by PCR. (G) Expression of p-PI3K and p-Akt was regulated by mir-320b and EGR3. Data are presented as the mean ± SD (n = 3 in each group). **p < 0.01 versus miR-320b+EGR3-NC group. ##p < 0.01 versus miR-320b+EGR3 group.