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CM double staining: (A) 10 cc of 0.05% crystal violet and 1 cc of 1% methylene blue is mixed in a 10 cc syringe (red arrow), and 1 cc of this mixture is aspirated in several different 10 cc syringes with 9 cc of air (yellow arrows). (B) Spraying of the CM mixture is done several times through the scope channel with an interval of 15 to 30 seconds until a satisfactory staining is achieved.
