REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-α-tubulin	Sigma-Aldrich	Cat# T5168; RRID: AB_477579
Mouse anti-Acetylated tubulin	Sigma-Aldrich	Cat# T7451; RRID: AB_609894
Rat anti-Tyrosinated tubulin	Abcam	Cat# Ab6160; RRID: AB_305328
Chicken anti-MAP2	Abcam	Cat# AB5392; RRID: AB_2138153
Chicken anti-GFP	Aves Labs	Cat# GFP-1020; RRID: AB_10000240
Rabbit anti-CAMSAP2	Proteintech	Cat# 17880-1-AP; RRID: AB_2068826
Rabbit anti-KIFC3	Santa Cruz	Cat# sc-134681; RRID: AB_10610803
Rabbit anti-TRIM46	[29]	N/A
Mouse anti-MAP2	Sigma-Aldrich	Cat# M9942; RRID: AB_477256
Rabbit anti-mCherry/RFP	Rockland	Cat# 600-401-379; RRID: AB_2209751
Mouse anti-mCherry	Clonetech	Cat# 632543; RRID: AB_2307319
Rabbit anti-GFP	Abcam	Cat# AB290; RRID: AB_2313768
anti-chicken Alexa405	Abcam	Cat# AB175675; RRID: AB_2810980
anti-rat Alexa488	Life Technologies	Cat# A11006; RRID: AB_2534074
anti-mouse Alexa488	Life Technologies	Cat# A11029; RRID: AB_2534088
anti-rabbit Alexa488	Life Technologies	Cat# A11034; RRID: AB_2576217
anti-chicken Alexa488	Thermo Fisher	Cat# A-11039; RRID: AB_142924
anti-mouse Alexa568	Life Technologies	Cat# A11031; RRID: AB_144696
anti-rabbit Alexa568	Life Technologies	Cat# A11036; RRID: AB_10563566
anti-rabbit Alexa647	Life Technologies	Cat# A21245; RRID: AB_2535813
anti-mouse Alexa647	Life Technologies	Cat# A21236; RRID: AB_2535805
Goat anti-rabbit IgG Antibody, IRDye 680LT Conjugated	LI-COR Biosciences	Cat# 827-11081; RRID: AB_10795015
Goat anti-mouse IgG Antibody, IRDye 680LT Conjugated	LI-COR Biosciences	Cat# 827-11080; RRID: AB_10795014
Goat anti-rabbit IgG Antibody, IRDye 800CW Conjugated	LI-COR Biosciences	Cat# 827-08365; RRID: AB_10796098
Goat anti-mouse IgG Antibody, IRDye 800CW Conjugated	LI-COR Biosciences	Cat# 827-08364; RRID: AB_10793856
Chemicals
FuGENE 6	Roche	Cat# 11836145001
Lipofectamine 2000	Thermo Fisher	Cat# 11668019
Vectashield mounting medium	Vectorlabs	Cat# H-1000
Doxycycline	Sigma-Aldrich	Cat# D1822
PEI	PolySciences	Cat# 24765–2
Rapalog	Takara	Cat# 635057
SIR-tubulin	Spiro Chrome	Cat# SC002
Critical Commercial Assays
Rat Neuron Nucleofector kit	Amaxa	Cat# VVPG-1003
Dynabeads M-280 streptavidin beads	Thermo Fisher	Cat# 11206D
Experimental Models: Cell Lines
African Green Monkey SV40-transformed kidney ATCC CRL-1651 fibroblast (COS-7)	ATCC	Cat# CRL-1651
Human embryonic kidney 239T (HEK293T)	ATCC	Cat# CRL-3216
Experimental Models: Organisms/Strains
Rat (Wistar)	Janvier	N/A
Mouse (C57 BL/6JRj)	Janvier	N/A
Recombinant DNA
Bio-mCherry	[30]	N/A
Bio-GFP-N1	[31]	N/A
pβactin-GFP	[32]	N/A
pGW1-GFP	[32]	N/A
pSuper vector	[33]	N/A
pβactin-GFP-FRB	[34]	N/A
pGW1-PEX-RFP-FKBP	[34]	N/A
Protein-biotin ligase BirA	[35]	N/A
GFP-MACF18	[10]	N/A
mCherry-α-tubulin	[10]	N/A
GFP-CAMSAP1	[10]	N/A
GFP-CAMSAP2	[10]	N/A
GFP-CAMSAP3	[10]	N/A
GFP-CAMSAP2-CC-CKK	[10]	N/A
GFP-CAMSAP2-N1	This paper	N/A
GFP-CAMSAP2-N2	This paper	N/A
GFP-CAMSAP2-N3	This paper	N/A
GFP-CAMSAP2-MBD-CKK	This paper	N/A
3GFP-CAMSAP2	[10]	N/A
Dox-inducible PA-GFP-α-tubulin	This paper	N/A
Bio-mCherry-KIFC3-CAMSAP2 chimera	This paper	N/A
Bio-mCherry-EMTB-CC-MD chimera	This paper	N/A
EMTB-3GFP	Addgene	Cat# 26741; RRID: Addgene_26741
Bio-mCherry-KIFC3-FL-WT	This paper	N/A
Bio-mCherry-KIFC3-N1	This paper	N/A
Bio-mCherry-KIFC3-N2	This paper	N/A
Bio-mCherry-KIFC3-N3	This paper	N/A
Bio-mCherry-KIFC3-N4	This paper	N/A
Bio-mCherry-KIFC3-N5	This paper	N/A
Bio-mCherry-KIFC3-CC-MD	This paper	N/A
Bio-mCherry-KIFC3-Rigor	This paper	N/A
Bio-mCherry-KIFC3-FL (mouse shRNAs resistant)	This paper	N/A
KIFC3-CC-MD-GFP-FRB	This paper	N/A
KIFC3-CC-MD-Rigor-GFP-FRB	This paper	N/A
Oligonucleotides
See Table S2.	N/A	N/A
Software and Algorithms
ImageJ	NIH	https://imagej.nih.gov/ij/; RRID: SCR_003070
NeuronJ	[36]	http://www.imagescience.org/meijering/software/neuronj/; RRID:SCR_002074
GraphPad Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/; RRID: SCR_002798
Kymoreslicewide	Github	https://github.com/ekatrukha/KymoResliceWide