Figure 4. TET3 Inhibition Reduces Liver Fibrosis.
(A) Masson and H&E staining on liver sections from mice treated with vehicle plus AAV8-scr, CCl4 plus AAV8-scr, or CCl4 plus AAV8-siTET3. Magnification, 4×; scale bars, 500 μm. Quantification of fibrosis from liver tissue sections was performed using ImageJ. Four sections per mouse (six mice per group) were counted. Fields were picked randomly with counting done in a blinded manner. Error bars are mean with SEM. **p < 0.01; significance by one-way ANOVA with Tukey post-test.
(B and C) qPCR (B) and IB (C) of indicated genes from liver tissues isolated from mice treated as in (A). RNA and protein extracted from liver tissues pooled from six animals per group were analyzed. Data are representative of three independent experiments. Error bars are mean with SEM of technical replicates (n = 3). *p < 0.05 and **p < 0.01; significance by one-way ANOVA with Tukey post-test.
(D) Hydroxyproline contents from liver tissues isolated from mice treated as in (A). Each data point represents an individual mouse, with six mice in each group.
(E) Serum ALP, ALT and bilirubin from mice treated as in (A). Each data point represents an individual mouse, with six mice in each group. *p < 0.05 and **p < 0.01; significance by one-way ANOVA with Tukey post-test.