| Antibodies |
| Anti-TET3 (Immunoblotting) |
GeneTex |
Cat# GTX121453 |
| Anti-TET1 (Immunoblotting) |
GeneTex |
Cat# GTX124207 |
| Anti-TET3 (ChIP) |
Millipore Sigma |
Cat# ABE290 |
| Anti-TGF-β1 |
Abcam |
Cat# ab92486 |
| Anti-TGFBR2 |
Abcam |
Cat# ab186838 |
| Anti-TSP1 |
Abcam |
Cat# ab85762 |
| Anti-SMAD3 |
Cell Signaling |
Cat# C67H9 |
| Anti-phospho-SMAD3 |
Cell Signaling |
Cat# C25A9 |
| Anti-COL1A1 |
Santa Cruz |
Cat# sc-293182 |
| Anti-FN1 |
Abcam |
Cat# ab194395 |
| Anti-α-SMA |
Abcam |
Cat# ab5694 |
| Anti-TIMP1 |
Cell Signaling |
Cat# 8946s |
| Anti-GAPDH |
Abcam |
Cat# ab128915 |
| Anti-HepPar1 |
Millipore Sigma |
Cat# 264M-94 |
| Anti-H3K4m3 (ChIP) |
Cell Signaling |
Cat# C42D8 |
| Anti-H3K27m3 (ChIP) |
Cell Signaling |
Cat# C36B11 |
| Anti-rabbit IgG, HRP-linked |
Cell Signaling |
Cat# 7074 |
| Anti-mouse IgG, HRP-linked |
Cell Signaling |
Cat# 7076 |
| Biological Samples |
| Human liver tissue blocks |
Yale Pathology Tissue Services |
https://medicine.yale.edu/pathology/ypts/ |
| Human primary hepatocytes and HSCs |
Liver Tissue Cell Distribution System |
N01-DK-7-0004/HHSN267200700004C |
| Chemicals, Peptides, and Recombinant Proteins |
| Human TGF-β1 |
Sigma-Aldrich |
Cat# T7039 |
| Glycochenodeoxycholic acid (GCDCA) |
Sigma-Aldrich |
Cat# G0759 |
| Dexamethasone |
Sigma-Aldrich |
Cat# D4902 |
| Insulin |
GIBCO |
Cat# 12585-014 |
| Williams Medium |
GIBCO |
Cat# 12551 |
| Carbon tetrachloride (CCl4) |
Sigma-Aldrich |
Cat# 319961 |
| Mineral oil |
Sigma-Aldrich |
Cat# M5310 |
| Critical Commercial Assays |
| EnVision G2 Doublestain System |
DAKO |
Cat# K5361 |
| Hydroxyproline Assay Kit |
Sigma-Aldrich |
Cat# MAK008-1KT |
| Bilirubin Assay Kit |
Sigma-Aldrich |
Cat# MAK126 |
| Pierce Agarose ChIP Kit |
Thermo Scientific |
Cat# 26156 |
| PrimeScript RT Reagent Kit |
TaKaRa |
RR037A |
| SYBR Green PCR Master Mix |
Bio-Rad |
172-5124 |
| PureLink RNA Mini Kit |
Ambion |
12183018A |
| Quick-gDNA MicroPrep |
Zymo Research |
D3021 |
| EZ DNA Methylation-Gold Kit |
Zymo Research |
D5006 |
| Experimental Models: Cell Lines |
| Human: LX-2 cells |
Sigma-Aldrich |
Cat# SCC064 |
| Experimental Models: Organisms/Strains |
| Mouse: C57B/6 (CCl4 model) |
Charles River |
Cat# 027 |
| Mouse: C57B/6J (BDL model) |
The Jackson Laboratory |
Cat# 000664 |
| Oligonucleotides |
| Primers for qPCR, ChIP, and QMSP see Table S1
|
This paper |
N/A |
| AAV8-siTET3: a cocktail of siRNAs targeting specifically to four different regions of mouse TET3: |
Applied Biological Materials |
Cat# iAAV04929608 |
| 5′-CGGTACCATCTCCTATTTCTCAGAGGGAG-3′ |
| 5′-GCCCACAAGGACCAACATAACCTCTACAA-3′ |
| 5′-CCTCTCCCTTTGCTCAGAGTTCCAGTTGC-3′ |
| 5′-GCCCTTGAGCTCCAACGAGAAGCTATTTG-3′ |
| AAV8-scr: a scrambled siRNAtargeting the following sequence: 5′-GGGTGAACTCACGTCAGAA-3′ |
Applied Biological Materials |
Cat# iAAV01500 |
| siTET3 (siRNA specific for human TET3) |
Ambion (Cao et al., 2019) |
Cat# 4392420/s47239 |
| siCon (nontargeting control siRNA) |
Ambion (Cao et al., 2019) |
Cat# AM4611 |
| Recombinant DNA |
| Plasmid: pcDNA-FLAG-Tet3 |
Addgene |
Cat# 60940 |
| Plasmid: pFLAG-CMV-2 (control vector) |
Sigma-Aldrich |
Cat# E7033 |
| Software and Algorithms |
| ImageJ |
|
https://imagej.nih.gov/ij/ |
| Prism 8 |
Graphpad |
https://www.graphpad.com/scientific-software/prism/ |
