KEY RESOURCES TABLE.
Where possible, we have listed resources using RRID numbers from the SciCrunch database.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Alexa Fluor 546-labeled goat anti-human | Invitrogen | RRID:AB_2535745 |
| Alexa Fluor 488-labeled goat anti-mouse | Invitrogen | RRID:AB_2534069 |
| Human anti-centromere antibodies (ACA) | Immunovision | RRID:AB_2744669 |
| mouse anti-β-tubulin | Sigma | RRID:AB_2827403 |
| SIRT2 | Sigma | RRID:AB_1079981 |
| SIRT1 | Cell Signaling Technologies | RRID:AB_2617130 |
| Actin | Millipore | RRID:AB_2223041 |
| HRP conjugated goat anti-rabbit IgG | Bio-Rad | RRID:AB_11125143 |
| HRP conjugated goat anti-mouse IgG | Bio-Rad | RRID:AB_11125547 |
| NMNAT1 | Santa Cruz | RRID:AB_2153131 |
| NMNAT3 | Santa Cruz | RRID:AB_2819344 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Hoescht 33342 (bis-benzimide 33342) | Sigma-Aldrich, Australia | 99403 |
| ECL Prime chemiluminescence detection system | GE Healthcare | RPN2232 |
| Nicotinamide mononucleotide | GeneHarbor Biotechnologies, Hong Kong | n/a |
| Pregnant mare’s serum gonadotrophin (Folligon) | MSD Animal Health, Australia Intervet, Boxmeer, Holland | |
| Triton X-100 | Sigma-Aldrich, Australia | T8787 |
| 3-isobutyl-1-methylxanthine | Sigma-Aldrich, Australia | I7018 |
| Paraffin oil | EMD Millipore, Australia | ES-005-C |
| M2 media | Sigma Aldrich, Australia | M7167 |
| Bovine serum albumin | Sigma Aldrich, Australia | |
| Monastrol | Sigma | M8515 |
| Sytox Green nucleic acid stain | Invitrogen | S7020 |
| HEPES-buffered α-minimum essential medium | GIBCO Life Technologies, Grand Island, NY | |
| human chorionic gonadotrophin | Chorulon, MSD Animal Health, Australia | |
| 5-(and-6)-Chloromethyl-2′,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) | Invitrogen | C6827 |
| Wash media (Research Wash) | IVF vet solutions (Adelaide, Australia) | |
| Cleavage media (Research Cleave) | IVF vet solutions (Adelaide, Australia) | |
| Experimental Models: Organisms/Strains | ||
| C57BL6/JAusb mice | Australian BioResources, Moss Vale | RRID:MGI:6200612 |
| SwissTacAusb mice | Australian BioResources, Moss Vale | |
| SIRT2 transgenic mice B6.Cg-Col1a1tm1(CAG-Sirt2)Jmi/ DsinJ), strain #029604 | Jackson Laboratory | RRID:IMSR_JAX:029604 |
| SIRT2 knockout mice (B6.129-Sirttm1.1Fwa/J), strain #012772 | Jackson Laboratory | RRID:IMSR_JAX:012772 |
| NMNAT1 transgenic mice | Obtained under material transfer agreement from NCPP, Japan. | RRID:IMSR_RBRC03166 |
| NMNAT3 transgenic mice | Obtained under material transfer agreement from NCPP, Japan. | RRID:IMSR_RBRC03167 |
| Software and Algorithms | ||
| NIS-Elements C acquisition and analysis software | Nikon, Japan | |
| ImageJ Fiji software | RRID:SCR_002285 | |
| Xcalibur software version 2.2, 2011 | ThermoFisher Scientific, Waltham MA | RRID:SCR_014593 |
| G*Power 3.1 | Heinrich Heine Universität Düsseldorf | RRID:SCR_013726 |
| Prism v8.1.2 | GraphPad | RRID:SCR_002798 |
| Other | ||
| A1 MP+ multiphoton confocal microscope | Nikon, Japan | |
| glass bottom 35 mm confocal dish | MatTek Corp. | |
| TSQAccess mass spectrometer | ThermoFisher Scientific, Waltham MA | |
| Prime 95B cMOS microscopy camera (multispectral imaging) | Photometries | |
| FluoroMax Plus-C fluorimeter (multispectral imaging microscope calibration) | Horiba | |
| Chow diet (mouse feed) | Gordon’s Specialty Stock Feeds, Yanderra, NSW, Australia | |
| Accu-check (hand-held glucose meter) | Roche | |
| EchoMRI (quantitative MRI, body composition) | EchoMRI, Houston TX, USA | |
| VisualSonics Vevo 2100 Ultrasound | VisualSonics | RRID:SCR_015816 |