Fig. 3.
JNK signaling is required for Toll pathway-triggered cell death. Micrographs showing Drosophila adult wings (a–h) and third instar larval wing discs (a′–h′, a′′–h′′). Compared with the controls (a–a′′), ectopic expression of Toll10B driven by Sd-Gal4 results in a small wing phenotype in adults (b), extensive cell death (b′) and apoptosis (b′′) in larval wing discs, which are suppressed by expressing a dominant-negative form of Bsk (c–c′′) or Puc (d–d′′). Depletion of cactus also produces a small wing phenotype in adults (e, g), massive cell death (e′, g′) and apoptosis (e′′, g′′) in larval wing discs, which are suppressed by expressing BskDN (f–f′′, h–h′′). i–k Statistical analysis of the adult wing size/wild type (WT) (i, n = 8), cell death number (j, n = 10) and apoptosis (k, n = 10) in wing discs are shown. One-way ANOVA was used to compute P-values, ****P < 0.0001, ***P < 0.001. See Additional file 1 for detailed genotypes. Scale bar: 100 μm