. 2020 Mar 10;11:19. doi: 10.1186/s40104-020-0427-5

Table 3.

Effects of in ovo feeding of L-arginine on digestive organ index of layer chicks¹

Item²			Arg dosage (mg/egg)			Pooled SEM	P-value
Item²	NC	SC	2	6	10	Pooled SEM	ANOVA	Linear³	Quadratic³
d 0 Index, %
Yolk sac	14.52^a	14.49^a	11.05^b	11.72^b	11.94^b	0.40	0.008	0.551	0.282
Proventriculus	0.53	0.56	0.66	0.54	0.60	0.02	0.086	0.656	0.910
Gizzard	4.01	4.35	4.48	4.23	4.53	0.08	0.211	0.352	0.800
Duodenum	16.34	15.70	15.56	15.77	16.15	0.20	0.721	0.294	0.602
Jejunum	28.16^b	28.68^b	34.49^a	29.61^b	31.79^ab	0.73	0.020	0.928	0.841
Ileum	25.40	25.54	26.34	25.40	25.99	0.64	0.989	0.910	0.896
d 3 Index, %
Yolk sac	2.67	2.91	1.93	1.89	1.85	0.16	0.088	0.065	0.159
Proventriculus	1.16	1.17	1.18	1.23	1.21	0.02	0.625	0.857	0.204
Gizzard	6.52	6.25	6.54	6.62	6.57	0.07	0.536	0.611	0.207
Duodenum	22.48^b	22.67^b	22.93^ab	24.71^ab	25.39^a	0.40	0.049	0.010	0.035
Jejunum	42.13	41.07	41.73	44.70	42.61	0.57	0.323	0.467	0.112
Ileum	31.23	32.98	34.75	36.36	33.92	0.74	0.224	0.803	0.157
d 14 Index, %
Proventriculus	0.77	0.71	0.76	0.80	0.74	0.01	0.100	0.546	0.131
Gizzard	3.63	3.57	3.68	3.62	3.66	0.05	0.971	0.674	0.924
Duodenum	10.70	10.65	9.39	9.22	10.71	0.40	0.606	0.822	0.272
Jejunum	19.02^b	19.21^b	22.52^a	22.05^a	23.66^a	0.45	0.001	0.025	0.039
Ileum	18.56	17.58	20.07	19.94	20.93	0.42	0.089	0.040	0.075
d 21 Index, %
Proventriculus	0.71	0.67	0.69	0.71	0.68	0.01	0.466	0.525	0.284
Gizzard	2.99	2.84	2.74	2.91	2.95	0.04	0.365	0.741	0.879
Duodenum	7.82	7.73	7.38	7.67	7.51	0.08	0.503	0.702	0.975
Jejunum	15.37	16.36	15.10	16.21	14.24	0.27	0.108	0.547	0.651
Ileum	14.51	15.92	14.54	15.17	13.24	0.30	0.109	0.777	0.305
d 42 Index, %
Proventriculus	0.69	0.71	0.63	0.72	0.71	0.02	0.308	0.422	0.578
Gizzard	2.60	2.47	2.44	2.57	2.43	0.05	0.740	0.866	0.701
Duodenum	4.13	3.85	3.88	4.10	3.97	0.05	0.194	0.328	0.229
Jejunum	8.43^b	7.89^b	7.77^b	8.04^b	9.21^a	0.13	0.001	0.031	0.042
Ileum	7.72^a	7.17^ab	6.58^b	7.54^a	7.80^a	0.12	0.004	0.235	0.103

^a–bMeans within a row with no common superscripts differ significantly (P < 0.05). ¹ Data are the mean of 8 replicates. NC, non-injected control group; SC, saline-injected control group.² Yolk sac and stomach index, % = absolute weight, g / body weight, g × 100, Intestinal index, % = absolute length, cm/ body weight, g × 100. ³Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing concentrations of L-arginine solution