Table 5.

Differential metabolites that mapped to pathway on day 3 post-hatch

Differential metabolites	Retention time	m/z	Fold changea	P-valueb	VIPc	Pathway
Stachyose	0.6476833	701.18808	0.420	0.007	3.454	Galactose metabolism
Raffinose	0.6476833	539.13622	0.555	0.004	2.501
Manninotriose	0.6476833	539.13622	0.555	0.004	2.501
Sucrose	0.6334000	377.08445	0.605	0.002	2.146
Taurine	0.5905500	124.00700	1.577	0.005	1.810	Primary bile acid biosynthesis
Taurochenodeoxycholate	7.2767833	498.28747	0.283	0.004	4.431
Taurodeoxycholate	8.2390167	498.28884	0.400	0.007	3.579
Tauroursodeoxycholic acid	6.9417667	498.28752	0.444	0.030	2.550
Linoleic acid	8.6883500	279.23228	0.796	0.042	1.408	Linoleic acid metabolism
Dihomo-γ-linolenic acid	9.5298333	305.24812	1.414	0.047	1.141	Linoleic acid metabolism
9,10-DHOA	8.4390333	313.23741	2.468	0.027	3.496	Linoleic acid metabolism
12,13-DHOA	7.5975333	313.23730	0.606	0.039	1.442	Linoleic acid metabolism
13,16,19-Docosatrienoic acid	9.8934500	333.27922	4.063	0.038	3.999	Lipid metabolism
DHA ethyl ester	9.5298333	355.26264	1.771	0.034	1.592
Amaranthin	0.6334000	761.14313	0.384	0.005	3.363	Secondary metabolites
Cucurbitacin D	9.4012667	515.30267	0.339	0.011	4.030

^aFold Change in the Arg10 group compared with the NC group. ^bP-values were calculated from Kruskal-Wallis test. ^cVIP (variable importance in the projection) values were obtained from partial least squares discriminant analysis models