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. Author manuscript; available in PMC: 2020 Mar 10.
Published in final edited form as: Cell Rep. 2020 Feb 11;30(6):2028–2039.e4. doi: 10.1016/j.celrep.2020.01.045

Figure 5. Calorie-Dependent Increase in VAN CART Expression Is Blunted in DIO.

Figure 5.

(A) Body weight gain (n = 6; Two-way ANOVA, interaction F(4,40) p<0.0001; Sidak’s multiple comparison **p<0.0079, ***p<0.0001) and (B) energy intake (n = 6; Two-way ANOVA, Interaction F(4,40) p = 0.0003; Sidak’s multiple-comparison test, *p = 0.0178, **p = 0.0054, ***p = 0.0002) of rats fed either HFHS or chow diet for 8 weeks.

(C) EIA quantification of CART protein concentration from both left and right NG from obese rats fasted 48 h or fasted and refed 2 h (n = 4; unpaired two-tailed t-test p = 0.078).

(D) The percent of CART+ neurons remains unchanged in response to 2 h refeeding in left or right NG (n = 4, two-way ANOVA, interaction F(1,12) = 0.9906, p = 0.34, Sidak’s post-hoc comparison).

(E) Representative stitched images of left or right NG CART expression from DIO rats either fasted 48 h or refed 2 h. Scale bar, 100 μm.

(F) NG CART concentration increased linearly with caloric intake in lean rats fed a HFHS meal (n = 10), but not in DIO rats (n = 7; differences in linear regression slopes F(1,13) = 119, p<0.0001).

(G) In DIO rats, intraNTS injection of CART antibody (2ng/ml) had no effect on caloric intake compared with saline (100 nl) in fasted or refed conditions (n=10/9; three-way ANOVA, F(4,4) = 0.12, p = 0.98). (H) 2 h food intake post CART antibody injection in fasted vs. fed DIO rats (n = 9/10; two-Way ANOVA, interaction F(1,8) = 0.27, p = 0.62).