Fig. 3.

Blockage of O-GlcNAc promotes autophagy flux. a – The 5637-GFP-LC3 cells were pretreated with Baf A1 (10 nM) for 24 h. The cells were then treated with DON for another 16 h. GFP-LC3 fluorescence was captured with fluorescence microscopy after the treatment. b – Baf A1 was added to 5637-GFP-LC3 cells stably transfected with sh-OGT or sh-OGA. GFP-LC3 fluorescence was captured with fluorescence microscopy. The average number of GFP-LC3 puncta was calculated for 200 cells. Data in the histograms are shown as the means ± SD. *P < 0.05 and **P < 0.01