Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 10;25:17. doi: 10.1186/s11658-020-00208-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — AMPK is O-GlcNAcylated. Co-IP was used to test the relationship between O-GlcNAc and AMPK. a – The 5637-GFP-AMPKα cells were treated with TG and cell extracts were used for the Co-IP with an anti-GFP antibody. IP products were subjected to detect the presence of O-GlcNAc (using western blotting with RL2 antibody) and that of AMPKα fused with GFP (using AMPKα antibody). b – The 5637 cells were transfected with an empty vector (Ctrl) or OGT-overexpression (OGT-OE) construct, and then the extracts were used for the Co-IP with an anti-GFP antibody and further analyzed using western blotting with RL2 antibody (left panel) or GFP and HA antibody (right panel)