Fig. 1.

P2X7-dependent increase in CD39 activity depends on lipid raft integrity in LPS-primed macrophages. (A,B) ATP hydrolysis (A) and AMP hydrolysis (B) in LPS-primed macrophages (1 µg/ml LPS for 4 h), stimulated with 500 µM ATP for 3 h that were then treated with 10 mM methyl-β-cyclodextrin (MβCD) or 50 mg/ml nystatin, for 10 min or benzyl alcohol (20 µM) for 30 min. One set of data is from cells treated with imipramine (30 µM) for 30 min before priming. Data are expressed as mean±s.e.m. of three independent experiments performed in triplicates. *P<0.05 when compared to unstimulated and untreated groups. ^#P<0.05 when non-treated groups were compared to drug-treated groups.