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Co-immunoprecipitation experiments were performed in triplicate. Pif1-myc is immunoprecipitated with an anti-Myc antibody (9E10). The presence of RPA in the Pif1-myc IP is monitored with an anti-Rfa1 or an anti-Rfa2 antibody. (+): cell extracts treated with benzonase. The asterisk (*) indicates a non-specific band. MW: molecular weight.
