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. 2020 Jan 17;4(3):48–63. doi: 10.15698/cst2020.03.214

Table 2.

Strains used in this study.

S. cerevisiae strains Genotype Origin
ORT6119-4 MATα CEB1-1.8 I-ARS305 Nicolas A.
ORT6135-36 MATα CEB1-1.8 II-ARS305 Nicolas A.
ORT6157-1 MATα CEB1-1.7 Gmut-ARS305 Nicolas A.
LM361 diploïd CEB1-1.8 I rfa1-D228Y/RFA1 pif1::Kan/PIF1 This study
LM411 diploïd CEB1-1.8 II rfa1-D228Y/RFA1 pif1::Kan/PIF1 This study
LM349 diploïd CEB1-1.7 Gmut rfa1-D228Y/RFA1 pif1::Kan/PIF1 This study
LM396 diploïd CEB1-1.8 I rfa1-D228Y/RFA1 + pVS45 (GAL::PIF1) This study
LM398 diploïd CEB1-1.8 II rfa1-D228Y/RFA1 + pVS45 (GAL::PIF1) This study
LM401 diploïd CEB1-1.8 I rfa1-D228Y/RFA1 + pSH380 (empty vector) This study
LM404 diploïd CEB1-1.8 II rfa1-D228Y/RFA1 + pSH380 (empty vector) This study
YVC600 diploïd CEB1-1.8 I mms1::TRP1/MMS1 This study
YVC601 diploïd CEB1-1.8 II mms1::TRP1/MMS1 This study
YVC602 diploïd CEB1-1.8 I rtt105::Kan/RTT105 This study
YVC603 diploïd CEB1-1.8 II rtt105::Kan /RTT105 This study
YVC604 diploïd CEB1-1.8 I rtt105::Kan/RTT105 GAL::RNH1::natMX/RNH1 This study
YVC605 diploïd CEB1-1.8 II rtt105::Kan/RTT105 GAL::RNH1::natMX /RNH1 This study
YVC606 diploïd CEB1-1.8 I pif1::Kan/PIF1 GAL::RNH1::natMX /RNH1 This study
YVC607 diploïd CEB1-1.8 I rfa1-D228Y/RFA1 GAL::RNH1::natMX /RNH1 This study
YVC608 diploïd CEB1-1.8 II rfa1-D228Y/RFA1 GAL::RNH1::natMX /RNH1 This study
LM140 MATα rfa1-D228Y Pif1-myc::KanMX6 This study
W1042-7C MATα can1-100,x SUP4-o::HIS3::pWJ317-CAN1-URA3 rfa1-D228Y Rothstein R
LM301 pif1::KanMX6 rfa1-D228Y This study
LM340 pif1::KanMX6 This study
YBL103 his3Δ1; leu2Δ0; ura3Δ0; met15Δ0; pGal-3HA-RNH1::NAT Luke B.
LM407 diploïd pGal-3HA-RNH1::natMX rfa1-D228Y/RFA1 This study
S. pombe strains Genotype Origin
JA961 h- leu1-32 ura4-D18 leu1+::pJK148-CEB25 orientation 1 This study
JA963 h- leu1-32 ura4-D18 leu1+::pJK148-CEB25-G12T orientation 1 This study
JA947 h- leu1-32 ura4-D18 leu1+::pJK148-CEB25 orientation 2 This study
JA948 h- leu1-32 ura4-D18 leu1+::pJK148-CEB25-G12T orientation 2 This study
SC387 h+ leu1-32 ura4-D18 ade6-M210 rad11-D223Y Ueno M.

All LM strains used are derivatives of W303-1B. All YVC strains used are derivatives of W303.

Spore colonies were generated from the diploid strains.