Table 2.
S. cerevisiae strains | Genotype | Origin |
ORT6119-4 | MATα CEB1-1.8 I-ARS305 | Nicolas A. |
ORT6135-36 | MATα CEB1-1.8 II-ARS305 | Nicolas A. |
ORT6157-1 | MATα CEB1-1.7 Gmut-ARS305 | Nicolas A. |
LM361 | diploïd CEB1-1.8 I rfa1-D228Y/RFA1 pif1::Kan/PIF1 | This study |
LM411 | diploïd CEB1-1.8 II rfa1-D228Y/RFA1 pif1::Kan/PIF1 | This study |
LM349 | diploïd CEB1-1.7 Gmut rfa1-D228Y/RFA1 pif1::Kan/PIF1 | This study |
LM396 | diploïd CEB1-1.8 I rfa1-D228Y/RFA1 + pVS45 (GAL::PIF1) | This study |
LM398 | diploïd CEB1-1.8 II rfa1-D228Y/RFA1 + pVS45 (GAL::PIF1) | This study |
LM401 | diploïd CEB1-1.8 I rfa1-D228Y/RFA1 + pSH380 (empty vector) | This study |
LM404 | diploïd CEB1-1.8 II rfa1-D228Y/RFA1 + pSH380 (empty vector) | This study |
YVC600 | diploïd CEB1-1.8 I mms1::TRP1/MMS1 | This study |
YVC601 | diploïd CEB1-1.8 II mms1::TRP1/MMS1 | This study |
YVC602 | diploïd CEB1-1.8 I rtt105::Kan/RTT105 | This study |
YVC603 | diploïd CEB1-1.8 II rtt105::Kan /RTT105 | This study |
YVC604 | diploïd CEB1-1.8 I rtt105::Kan/RTT105 GAL::RNH1::natMX/RNH1 | This study |
YVC605 | diploïd CEB1-1.8 II rtt105::Kan/RTT105 GAL::RNH1::natMX /RNH1 | This study |
YVC606 | diploïd CEB1-1.8 I pif1::Kan/PIF1 GAL::RNH1::natMX /RNH1 | This study |
YVC607 | diploïd CEB1-1.8 I rfa1-D228Y/RFA1 GAL::RNH1::natMX /RNH1 | This study |
YVC608 | diploïd CEB1-1.8 II rfa1-D228Y/RFA1 GAL::RNH1::natMX /RNH1 | This study |
LM140 | MATα rfa1-D228Y Pif1-myc::KanMX6 | This study |
W1042-7C | MATα can1-100,x SUP4-o::HIS3::pWJ317-CAN1-URA3 rfa1-D228Y | Rothstein R |
LM301 | pif1::KanMX6 rfa1-D228Y | This study |
LM340 | pif1::KanMX6 | This study |
YBL103 | his3Δ1; leu2Δ0; ura3Δ0; met15Δ0; pGal-3HA-RNH1::NAT | Luke B. |
LM407 | diploïd pGal-3HA-RNH1::natMX rfa1-D228Y/RFA1 | This study |
S. pombe strains | Genotype | Origin |
JA961 | h- leu1-32 ura4-D18 leu1+::pJK148-CEB25 orientation 1 | This study |
JA963 | h- leu1-32 ura4-D18 leu1+::pJK148-CEB25-G12T orientation 1 | This study |
JA947 | h- leu1-32 ura4-D18 leu1+::pJK148-CEB25 orientation 2 | This study |
JA948 | h- leu1-32 ura4-D18 leu1+::pJK148-CEB25-G12T orientation 2 | This study |
SC387 | h+ leu1-32 ura4-D18 ade6-M210 rad11-D223Y | Ueno M. |
All LM strains used are derivatives of W303-1B. All YVC strains used are derivatives of W303.
Spore colonies were generated from the diploid strains.