Figure 3.

Isoliquiritigenin (ILQ) decreased the transcriptional activity of AP-1. A-B, ILQ inhibited the expression of AP-1. KYSE-140 and TE-1 cells were treated with 10 μM ILQ, and the expression of the indicated protein was examined by western blotting. C-D, ILQ decreased the transcriptional activity of AP-1. The pGL3-AP-1 vector was transfected into KYSE-140 or TE-1 cell and then treated with ILQ for 24 h; the transcriptional activity of AP-1 was detected as described. E, EGFR knockdown attenuated the inhibitory effect of ILQ on AP-1. KYSE-140 cells were infected with EGFR-shRNA and then transfected with pGL3-AP-1 vector; after the treatment of ILQ, the transcriptional activity of AP-1 was determined as described.