Figure 5.

Effects of CCL3 deficiency on macrophages infection ratio and production of NO_x and cytokines. (A) Peritoneal macrophages from ccl3^+/+ or ccl3^−/− mice were harvested and allowed to adhere on coverslips (2 h), pre-treated or not treated with IFNγ (2 h) and infected (4 h) by trypomastigote forms of the Colombian strain (MOI of 10:1). (B) Percentages of infected cells were quantified. (C) Concentrations of NO_x in culture supernatants were determined by Griess reagent. Concentrations of (D) TNF and (E) IL-10 were evaluated using ELISA. Results are representative of three independent experiments performed in triplicates. Data are presented as means ± SE. *p < 0.05 and **p < 0.01, ***p < 0.001, comparing infected with non-infected cells or comparing IFN-treated with not treated. ^##p < 0.01 and ^###p < 0.001, comparing T. cruzi-infected ccl3^+/+ and ccl3^−/− macrophages or infected or non-infected cells. ^&&p < 0.01, ^&&&p < 0.01. (ANOVA Bonferroni posttest, t-Student test).