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. 2020 Mar 3;11:306. doi: 10.3389/fimmu.2020.00306

Figure 6.

Figure 6

Effects of CCL3 deficiency on CCC main features: heart geometry alterations, loss of LVEF, QTc prolongation and inflammatory/regulatory cytokine unbalance. ccl3+/+ and ccl3−/− mice were infected with 100 trypomastigote forms of the Colombian T. cruzi strain and analyzed at 120 dpi. (A) Relative heart weight (heart weight in mg/g body) (B) Representative pictures of hearts of NI controls and T. cruzi-infected ccl3+/+ and ccl3−/− mice. Bars = 1 cm. (C) Heart longitudinal axis (mm) in systole (s) was obtained by echocardiography. (D) Percentage of left ventricular ejection fraction (LVEF) was determined by echocardiography. (E) CK-MB activity in serum determined by biochemical assay. Data represent two independent experiments with three non-infected (F) Group data for the ECG records showing QTc intervals (ms). (G) Extracts of hearts were prepared and TNF and IL-10 concentrations were estimated by ELISA. Horizontal gray bars show cytokine levels in sex- and age-matched NI control mice (means) and four to five infected mice per group. Data represent two independent experiments. The data are represented as means ± SE. *p < 0.05, **p < 0.01, ***p < 0.001, comparing T. cruzi-infected and NI mice; #p < 0.05, ##p < 0.01, ###p< 0.001, comparing T. cruzi-infected ccl3+/+ and ccl3−/− mice. (t-Student test, ANOVA Bonferroni posttest).